Membrane Proteins

Summary

Ann McDermott’s research group studies the structure, flexibility, and function of proteins using magnetic resonance methods. For example, we study the structure and function of potassium ion channels, which play crucial roles in diverse contexts, from bacteria to the human nervous system. Our work illuminated the role of an ion-depleted state and provided evidence for strong allosteric coupling in channel regulation, using the prototypical prokaryotic ion channel KcsA. As for many enzymes and drug targets, ligand binding is associated with internal motions. The characteristic timescales of active-site flexible loop motions in other proteins have been studied in the past by the McDermott group, and ongoing studies of the channel position us to carry out detailed dynamics of the channel.

People

McDermott Group Publications

  1. Xu, Yunyao. , Bhate, M.. and McDermott, Ann. E. "Transmembrane allosteric energetics characterization for strong coupling between proton and potassium ion binding in the KcsA channel" 2017. Proceedings of the National Academy of Sciences of the United States of America 114 :8788-8793 link info
  2. Laage, S.. , Tao, Y.. and McDermott, Ann. E. "Cardiolipin interaction with subunit c of ATP synthase: Solid-state NMR characterization" 2015. Biochimica et Biophysica Acta - Biomembranes 1848 :260-265 link info
  3. Wylie, B.. , Bhate, M.. and McDermott, Ann. E. "Transmembrane allosteric coupling of the gates in a potassium channel" 2014. Proceedings of the National Academy of Sciences of the United States of America 111 :185-190 link info
  4. Bhate, M.. , Wylie, B.. , Thompson, A.. , Tian, L.. , Nimigean, C.. and McDermott, Ann. E. "Preparation of uniformly isotope labeled KcsA for solid state NMR: Expression, purification, reconstitution into liposomes and functional assay" 2013. Protein Expression and Purification 91 :119-124 link info
  5. Catalano, J.. , Sadre-Bazzaz, K.. , Amodeo, G.. , Tong, L.. and McDermott, Ann. E. "Structural evidence: A single charged residue affects substrate binding in cytochrome P450 BM-3" 2013. Biochemistry 52 :6807-6815 link info
  6. Bhate, M.. and McDermott, Ann. E. "Protonation state of E71 in KcsA and its role for channel collapse and inactivation" 2012. Proceedings of the National Academy of Sciences of the United States of America 109 :15265-15270 link info
  7. Bauer, A.. , Gieschler, S.. , Lemberg, K.. , McDermott, Ann. E. and Stockwell, B.. "Functional model of metabolite gating by human voltage-dependent anion channel 2" 2011. Biochemistry 50 :3408-3410 link info