Dynamic nuclear polarization


Dynamic nuclear polarization (DNP) is a technique for transferring polarization from unpaired electrons to nearby nuclei to enhance the signal-to-noise ratio of the NMR spectra. We develop novel methods to apply DNP to membrane proteins and viruses. In addition to using DNP to enable studies of complex systems such as KcsA and the Pf1 bacteriophage, ongoing investigations in the McDermott group are specifically labeling proteins with radicals in order to use them both as polarizing agents and structural probes


McDermott Group Publications

  1. Fritzsching, Keith. J. , Itin, B.. and McDermott, Ann. E. # "N,N-Diethylmethylamine as lineshape standard for NMR above 130 K" 2018. Journal of Magnetic Resonance 287 :110-112 link info
  2. Rogawski, Rivkah. , Sergeyev, I.. , Li, Y.. , Ottaviani, M.. , Cornish, V.. and McDermott, Ann. E. "Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags" 2017. Journal of Physical Chemistry B 121 :1169-1175 link info
  3. Sergeyev, I.. , Itin, B.. , Rogawski, Rivkah. , Day, L.. and McDermott, Ann. E. "Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization" 2017. Proceedings of the National Academy of Sciences of the United States of America 114 :5171-5176 link info
  4. Rogawski, Rivkah. and McDermott, Ann. E. "New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR" 2017. Archives of Biochemistry and Biophysics 628 :102-113 link info
  5. Wylie, B.. , Dzikovski, B.. , Pawsey, S.. , Caporini, M.. , Rosay, M.. , Freed, J.. and McDermott, Ann. E. "Dynamic nuclear polarization of membrane proteins: Covalently bound spin-labels at protein-protein interfaces" 2015. Journal of Biomolecular NMR 61 :361-367 link info